经典实验室常用缓冲液配置方案

2023年12月17日发(作者：丰田汉兰达图片及价格)

Delegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other实验室常用缓冲液配置方案

1)1 M Tris-HCl （pH7.4， 7。6, 8。0)

组份浓度：1 M Tris—HCl

配制量：1 L

配制方法：

1。 称量121.1 g Tris置于1 L烧杯中.

2。 加入约800 ml的去离子水，充分搅拌溶解.

3. 按下表量加入浓盐酸调节所需要的pH值。

PH值

7。4 约70ml

7。6 约60ml

8.0

4. 将溶液定容至1 L.

5. 高温高压灭菌后，室温保存。

注意:应使溶液冷却至室温后再调定pH值，因为Tris溶液的pH值随温度的变化差异很大,温度每升高1℃，溶液的pH值大约降低0.03个单位。

2)10×TE Buffer（pH7。4， 7.6, 8。0）

组份浓度:100 mM Tris-HCl， 10 mM EDTA

配制量：1 L

配制方法：

1。 量取下列溶液，置于1 L烧杯中。

1M Tris－HCl Buffer（PH7。4，7.6，8。0)

500mM EDTA(PH8.0）

2. 向烧杯中加入约800 ml的去离子水，均匀混合。

3。 将溶液定容至1 L后，高温高压灭菌。

4. 室温保存.

3）1。5 M Tris—HCl (pH8.8）

组份浓度：1.5 M Tris-HCl

配制量：1 L

配制方法：

1。 称量181。7 g Tris置于1 L烧杯中。

2. 加入约800 ml的去离子水，充分搅拌溶解。

3. 用浓盐酸调节pH值至8。8.

4。 将溶液定容至1 L.

5。 高温高压灭菌后,室温保存。

注意:应使溶液冷却至室温后再调定pH值，因为Tris溶液的pH值随温度的变化差异很大，温度每升高1℃，20ml

100ml

约42ml

浓HCl

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreachDelegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other溶液的pH值大约降低0.03个单位。

4）3 M醋酸钠（pH5。2）

组份浓度:3M 醋酸钠

配制量：100ml

配制方法：

1。称量40.8g NaAc·3H2O置于100-200ml烧杯中，加入月40ml的去离子水搅拌溶解

2。加入冰醋酸调节pH值至5.2

3。加去离子水将溶液定容至100ml

4高温高压灭菌后，室温保存。

5)PBS Buffer

组份浓度:137 mM NaCl， 2.7 mM KCl， 10 mM Na2HPO4, 2 mM KH2PO4

配制量:1 L

配制方法：

1。 称量下列试剂,置于1 L烧杯中。

NaCl

KCl

Na2HPO4

8g

0.2g

1.42g

KH2PO4

0.27g

2. 向烧杯中加入约800 ml的去离子水，充分搅拌溶解.

3. 滴加浓盐酸将pH值调节至7。4,然后加入去离子水将溶液定容至1 L。

4. 高温高压灭菌后，室温保存。

注意：上述PBS Buffer中无二价阳离子，如需要，可在配方中补充1 mM CaCl2和0。5 mM MgCl2。

6)10 M 醋酸铵

组份浓度：10 M 醋酸铵

配制量：100 ml

配制方法:

1。 称量77.1 g醋酸铵置于100~200 ml烧杯中，加入约30 ml的去离子水搅拌溶解。

2。 加去离子水将溶液定容至100 ml。

3。 使用0.22 mm滤膜过滤除菌。

4. 密封瓶口于室温保存。

注意:醋酸铵受热易分解，所以不能高温高压灭菌.

7)苯酚/氯仿/异戊醇(25 : 24 : 1)

配制方法：

1。 说明:从核酸样品中除去蛋白质时常常使用苯酚/氯仿/异戊醇(25 : 24 ： 1)。氯仿可使蛋白质变性并有助于液相与有机相的分离，而异戊醇则有助于消除抽提过程中出现的气泡。

2. 配制方法：将Tris-HCl平衡苯酚与等体积的氯仿/异戊醇（24 ： 1)混合均匀后，移入棕色玻璃瓶中4℃保存。

8）10% (W/V) SDS

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreach Delegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other组份浓度：10％ (W/V）SDS

配制量：100ml

配制方法：

1。称量10g高纯度的SDS置于100—200ml烧杯中，加入约80ml的去离子水,68℃加入溶解

2.滴加浓盐酸调节pH值至7。2

3.将溶液定容至100ml后，室温保存。

9）2 N NaOH

组份浓度:2 N NaOH

配制量：100 ml

配制方法:

1。 量取80 ml去离子水置于100~200 ml塑料烧杯中(NaOH溶解过程中大量放热,有可能使玻璃烧杯炸裂)。

2。 称取8 g NaOH小心地逐渐加入到烧杯中,边加边搅拌。

3. 待NaOH完全溶解后，用去离子水将溶液体积定容至100 ml。

4. 将溶液转移至塑料容器中后，室温保存。

10）2。5 N HCl

组份浓度:2。5 N HCl

配制量：100 ml

配制方法：

1. 在78。4 ml的去离子水中加入21.6 ml的浓盐酸（11.6 N）,均匀混合。

2。 室温保存。

11）5 M NaCl

组份浓度：5 M NaCl

配制量：1 L

配制方法：

1。 称取292.2 g NaCl置于1 L烧杯中，加入约800 ml的去离子水后搅拌溶解。

2. 加去离子水将溶液定容至1 L后，适量分成小份.

3。 高温高压灭菌后，4℃保存。

11）20％ (W/V) Glucose

组份浓度：20％ （W/V） Glucose

配制量:100 ml

配制方法：

1. 称取20 g Glucose置于100～200 ml烧杯中，加入约80 ml的去离子水后,搅拌溶解。

2。 加去离子水将溶液定容至100 ml。

3. 高温高压灭菌后,4℃保存。

12）SolutionI（质粒提取用)

组份浓度：25 mM Tris—HCl（pH8.0)， 10 mM EDTA, 50 mM Glucose

配制量：1 L

配制方法：

1。 量取下列溶液，置于1 L烧杯中。

1M

Tris—HCl（PH8.0）

0。5M 20ml

EDTA25ml

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreachDelegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other（PH8。0）

20%Glucose（1。11M）

dH2O

2。 高温高压灭菌后,4℃保存。

3. 使用前每50 ml的Solution I中加入2 ml的RNase A（20 mg/ml）.

13）SolutionII（质粒提取用）

组份浓度：200mM NaOH,1％(W/V）SDS

配制量:500ml

配制方法：

1。量取下列溶液，置于500ml烧杯中

10％ SDS 50ml

2N NaOH 50ml

2。加灭菌水定容至500ml，充分混匀

3.室温保存,此溶液保存时间最好不要超过一个月

注意：SDS易产生气泡，不要剧烈搅拌

14）SolutionIII（质粒提取用）

组份浓度:3 M KOAc， 5 M CH3COOH

配制量：500 ml

配制方法：

1. 称量下列试剂，置于500 ml烧杯中。

KOAc 147g

CH3COOH

2。 加入300 ml去离子水后搅拌溶解。

3. 加去离子水将溶液定容至500 ml。

4。 高温高压灭菌后,4℃保存。

15）0。5 M EDTA（pH8。0）

组份浓度：0.5 M EDTA

配制量:1 L

配制方法:

称取186。1 g Na2EDTA·2H2O，置于1 L烧杯中。

2. 加入约800 ml的去离子水，充分搅拌.

3. 用NaOH调节pH值至8。0（约20 g NaOH）.

注意：pH值至8.0时，EDTA才能完全溶解。

4。 加去离子水将溶液定容至1 L。

5。 适量分成小份后，高温高压灭菌。

6. 室温保存.

16）1 M DTT

组份浓度：1 M DTT

配制量：20 ml

57。5ml

910ml

45ml

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreach Delegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other配制方法：

1。 称取3.09 g DTT，加入到50 ml塑料离心管内。

2. 加20 ml的0.01 M NaOAc（pH5。2)，溶解后使用0。22 mm滤器过滤除菌。

3。 适量分成小份后，—20℃保存。

17）10 mM ATP

组份浓度：10 mM ATP

配制量：20 ml

配制方法：

1。 称取121 mg Na2ATP·3H2O,加入到50 ml塑料离心管内.

2. 加20 ml的25 mM Tris-HCl（pH8。0），搅拌溶解。

3. 适量分成小份后，-20℃保存.

一.常用贮液与溶液

1mol/L亚精胺（Spermidine): 溶解2.55g亚精胺于足量的水中,使终体积为10ml。分装成小份贮存于-20℃。

1mol/L精胺（Spermine）：溶解3。48g精胺于足量的水中，使终体积为10ml.分装成小份贮存于-20℃。

10mol/L乙酸胺（ammonium acetate）：将77.1g乙酸胺溶解于水中，加水定容至1L后,用0.22um孔径的滤膜过滤除菌。

10mg/ml牛血清蛋白(BSA）：加100mg的牛血清蛋白（组分V或分子生物学试剂级,无DNA酶）于9。5ml水中（为减少变性，

须将蛋白加入水中,而不是将水加入蛋白），盖好盖后，轻轻摇动，直至牛血清蛋白完全溶解为止.不要涡旋混合。加水定容到10ml,然后分装成小份贮存于—20℃.

1mol/L二硫苏糖醇（DTT）:在二硫苏糖醇5g的原装瓶中加32。4ml水，分成小份贮存于-20℃。或转移100mg的二硫苏糖醇

至微量离心管,加0.65ml的水配制成1mol/L二硫苏糖醇溶液。

8mol/L乙酸钾（potassium acetate）：溶解78。5g乙酸钾于足量的水中，加水定容到100ml.

1mol/L氯化钾(KCl）：溶解7。46g氯化钾于足量的水中,加水定容到100ml。

3mol/L乙酸钠（sodium acetate）：溶解40。8g的三水乙酸钠于约90ml水中,用冰乙酸调溶液的pH至5.2，再加水定容到100ml。

0。5mol/L EDTA：配制等摩尔的Na2EDTA和NaOH溶液（0.5mol/L），混合后形成EDTA的三钠盐。或称取186.1g的Na2EDTA·2H2O和20g的NaOH，并溶于水中，定容至1L。

1mol/L HEPES：将23。8gHEPES溶于约90ml的水中，用NaOH调pH（6。8—8.2），然后用水定容至100ml。

1mol/L HCl：加8.6ml的浓盐酸至91.4ml的水中。

25mg/ml IPGT：溶解250mg的IPGT(异丙基硫代—β—D-半乳糖苷）于10ml水中，分成小份贮存于-20℃。

1mol/LMgCl2:溶解20.3g MgCl2·6H2O于足量的水中，定容到100ml。

100mmol/L PMSF：溶解174mg的PMSF（苯甲基磺酰氟）于足量的异丙醇中，定容到10ml.分成小份并用铝箔将装液管包裹

或贮存于-20℃。

20mg/ml蛋白酶K（proteinase K）:将200mg的蛋白酶L加入到9。5ml水中，轻轻摇动，直至蛋白酶K完全溶解。不要涡旋混合。加水定容到10ml,然后分装成小份贮存于-20℃。

10mg/mlRnase(无DNase）（DNase－free RNase）：溶解10mg的胰蛋白RNA酶于1ml的10mmol/L的乙酸钠水溶液中（pH 5.0）。溶解后于水浴中煮沸15min，使DNA酶失活。用1mol/L的Tris－HCl调pH至7。5,于—20℃贮存。（配制过程中要戴手套）

5mol/L氯化钠（NaCl）:溶解29.2g氯化钠于足量的水中，定容至100ml。

10N氢氧化钠(NaOH）：溶解400g氢氧化钠颗粒于约0。9L水的烧杯中(磁力搅拌器搅拌)，氢氧化钠完全溶解后用水定容至1L。

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreachDelegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other10％SDS(十二烷基硫酸钠）:称取100gSDS慢慢转移到约含0。9L的水的烧杯中，用磁力搅拌器搅拌直至完全溶解。用水定容至1L。

2mol/L山梨（糖)醇（Sorbitol)：溶解36。4g山梨（糖）醇于足量水中使终体积为100ml。

100％三氯乙酸（TCA）：在装有500gTCA的试剂瓶中加入100ml水，用磁力搅拌器搅拌直至完全溶解。（稀释液应在临用前配制）

2.5％ X－gal（5—溴—4-氯—3—吲哚－β—半乳糖苷）：溶解25mg的X－gal于1ml的二甲基甲酰胺（DMF),用铝箔包裹装液管，贮存于—20℃。

100×Denhardt试剂(Denhardt\'s regent）

成分及终浓度

2％聚蔗糖（Ficoll，400型）

2％聚乙烯吡咯烷酮（PVP—40）

2％BSA（组分V）

水

依照上表称取各组分，溶于水中定容。过滤除菌及杂质，分装成小份于—20℃贮存。

10×标准DNA连接酶缓冲液（standard DNA ligase buffer)（粘端、平端连接）

成分及终浓度

0。5mol/L Tris-HCl

100mmol/L MgCl2

100mmol/L DTT

2mmol/L ATP

5mmol/L 盐酸亚精胺(可选)

水

将配制好的缓冲液分装成小份，贮存于—20℃ 。

100 mmol/L dNTP 溶液（dNTP solutions)

可以购买到100mmol/L纯dNTPs贮液，-80℃可贮存至少6个月。

10mmol/L dNTP混合液

成分及终浓度

10mmol/L dATP

10mmol/L dCTP

10mmol/L dGTP

10mmol/L dTTP

水

20％PEG 8000/2.5M NaCl

成分及终浓度

质量浓度为20%聚乙配制10ml溶液各成分的用量

20g

配制20ul溶液各成分的用量

2ul 100 mmol/L dATP 贮液

2ul 100 mmol/L dCTP 贮液

2ul 100 mmol/L dGTP 贮液

2ul 100 mmol/L dTTP 贮液

12ul

配制10ml溶液各成分的用量

5ml 1mol/L 贮液

1ml 1mol/L 贮液

1ml 1mol/L 贮液

200ul 100 mmol/L 贮液

50ul 1 mmol/L 贮液

2。25ml

配制100ml溶液各成分的用量

2g

2g

2g

加水至总体积为100ml

0。5mg/ml BSA(组分V）（可选) 0.5ml 10 mg/mL 贮液

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreach Delegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other二醇

2。5mol/L 氯化钠

水

50ml 5 mol/L 氯化钠 或 14。6g 固体氯化钠

补足100ml

加聚乙二醇于含有氯化钠的烧杯中,加水至终体积100ml，用磁力搅拌器搅拌溶解。

20×SSC

成分及终浓度

300mmol/L 柠檬酸三钠（二水）

3mol/L 氯化钠

水

溶解柠檬酸三钠(二水）和氯化钠于约0.9L水中，加几滴10N NaOH溶液调pH为7。0，用水补足体积至1L。

DEPC（焦碳酸二乙酯）处理水

加100ul DEPC 于 100ml 水中，使DEPC的体积分数为0.1%。在37℃温浴至少12h,然后在15 psi 条件下高压灭菌20min,以使残余的DEPC失活。DEPC会与胺起反应，不可用DEPC处理Tris缓冲液。

甲酰胺（deionized formamide）

直接购买或加Dowex XG8 混合树脂于装有甲酰胺的玻璃烧杯中，用磁力搅拌器轻轻搅拌1h,可去除甲酰胺中的离子。经Whatman 1号滤纸过滤除去树脂后分成小份，充氮气于—80℃贮存(防止氧化）.

磷酸缓冲液（phosphate buffer)

按照下表所给定的体积,混合1 mol/L 的磷酸二氢钠（单碱）和1mol/L 磷酸氢二钠（双碱)贮液，获得所需pH的磷酸缓冲液。配制1 mol/L 的磷酸二氢钠（NaH2PO4·H2O)贮液：溶解138g于足量水中,使终体积为1L；1mol/L

磷酸氢二钠(Na2HPO4）贮液：溶解142g于足量水中使终体积为1L.

1mol/L 磷酸二氢钠（ml）

877

850

815

775

735

685

625

565

510

450

390

330

280

1mol/L 磷酸氢二钠(ml）

123

150

185

225

265

315

375

435

490

550

610

670

720

最终pH值

6.0

6。1

6。2

6。3

6。4

6。5

6。6

6.7

6。8

6。9

7.0

7.1

7.2

配制1L溶液各成分的用量

88.2g

175。3g

补足1L

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreachDelegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and otherTE（用于悬浮和贮存DNA）

成分及终浓度

10mmol/L Tris－HCl

1mmol/L EDTA

水

Tris缓冲液(Tris—HCl buffer)

将121g的Tris碱溶解于约0。9L水中，再根据所要求的pH（25℃下)加一定量的浓盐酸（11。6N），用水调整终体积至1L。

浓盐酸的体积（ml）

8。6

14

21

28.5

38

46

56

66

71.3

76

二。电泳缓冲液、染料和凝胶加样液

电泳缓冲液

50×Tris-乙酸（TAE)缓冲液

成分及终浓度

2mol/L Tris碱

1mol/L 乙酸

100 mmol/L EDTA

水

5×Tris-硼酸(TBE)缓冲液

成分及终浓度

445 mmol/L Tris碱

445 mmol/L 硼酸盐

10 mmol/L EDTA

水

染料

1％溴酚蓝（bromophenol blue）

加1g水溶性钠型溴酚蓝于100ml水中，搅拌或涡旋混合直到完全溶解。

配制1L溶液各成分的用量

54g

27。5g 硼酸

20 ml的0。5 mol/L EDTA(pH 8.0）

补足1L

配制1L溶液各成分的用量

242g

57.1 ml的冰乙酸（17。4 mol/L）

200ml的0。5 mol/L EDTA（pH 8。0）

补足1L

pH

9.0

8。8

8.6

8。4

8.2

8。0

7。8

7.6

7.4

7。2

配制100ml溶液各成分的用量

1ml 1mol/L Tris-HCl（pH7.4—8。0，25℃）

200ul 0.5 mol/L EDTA（pH 8。0）

98。8ml

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreach Delegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other1％二甲苯青FF（xylene cyanole FF）

溶解1g二甲苯青FF于足量水中，定容到100ml。

10mg/ml的溴化乙锭（ethidium bromide）

小心称取1g溴化乙锭，转移到广口瓶中，加100ml水，用磁力搅拌器搅拌直到完全溶解.用铝箔包裹装液管，于4℃贮存。

凝胶上样液（gel loading solutions）

6×碱性凝胶上样液（室温贮存）

成分及终浓度

0.3 N 氢氧化钠

6 mmol/L EDTA

18％聚蔗糖（400型）

0。15％溴甲酚绿

0。25％二甲苯青FF

水

6×聚蔗糖凝胶上样液(室温贮存）

成分及终浓度

0。15％溴酚蓝

0.15%二甲苯青FF

5 mmol/L EDTA

15％聚蔗糖（400型）

水

6×溴酚蓝/二甲苯青/聚蔗糖凝胶上样液（室温贮存）

成分及终浓度

0.25％溴酚蓝

0。25%二甲苯青FF

15%聚蔗糖（400型）

水

6×甘油凝胶上样液（4℃贮存）

成分及终浓度

0.15%溴酚蓝

0。15％二甲苯青FF

5 mmol/L EDTA

50％甘油

水

6×蔗糖凝胶上样液（室温贮存）

成分及终浓度 配制10ml溶液各成分用量

配制10ml溶液各成分用量

1。5ml 1％溴酚蓝

1。5ml 1％二甲苯青FF

100ul 0。5mol/L EDTA（pH8.0）

3ml

3。9ml

配制10ml溶液各成分用量

2.5ml 1％溴酚蓝

2.5ml 1％二甲苯青FF

1.5g

补足到10ml

配制10ml溶液各成分用量

1。5ml 1％溴酚蓝

1。5ml 1％二甲苯青FF

100ul 0.5mol/L EDTA（pH8。0）

1.5g

补足到10ml

配制10ml溶液各成分用量

300ul 10N 氢氧化钠

120ul 0。5mol/L EDTA（pH8。0)

1。8g

15mg

25mg

补足到10ml

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreachDelegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other0。15％溴酚蓝

0.15％二甲苯青FF

5 mmol/L EDTA

40％聚蔗糖

水

1。5ml 1％溴酚蓝

1.5ml 1％二甲苯青FF

100ul 0。5mol/L EDTA（pH8。0）

4g

补足到10ml

10×十二烷基硫酸钠/甘油凝胶上样液(室温贮存）

成分及终浓度

0。2％溴酚蓝

0。2％二甲苯青FF

200 mmol/L EDTA

0.1%SDS

50％甘油

水

配制10ml溶液各成分用量

20mg

20mg

4ml 0。5mol/L EDTA（pH8。0）

100ul 10％ SDS

5ml

补足到10ml

三.常用培养基

LB培养基

将下列组分溶解在0.9L水中：

蛋白胨

酵母提取物

氯化钠

如果需要用1N NaOH(～1ml)调整pH至7。0，再补足水至1L。注：琼脂平板需添加琼脂粉12g/L，上层琼脂平板添加琼脂粉7g/L。

SOB培养基

将下列组分溶解在0。9L水中：

蛋白胨

酵母提取物

氯化钠

1 mol/L 氯化钾

用水补足体积到1L。分成100ml的小份,高压灭菌。培养基冷却到室温后，再在每100ml的小份中加1ml灭过菌的1mol/L氯化镁。

SOC培养基

成分、方法同SOB培养基的配制，只是在培养基冷却到室温后，除了在每100ml的小份中加1ml灭过菌的1mol/L氯化镁外，再加2ml灭菌的1mol/L葡萄糖（18g葡萄糖溶于足够水中，再用水补足到100ml,用0.22um的滤膜过滤除菌)。

TB培养基

将下列组分溶解在0。9L水中:

20g

5g

0.5g

2.5ml

10g

5g

10g

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreach Delegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other蛋白胨

酵母提取物

甘油

12g

24g

4ml

各组分溶解后高压灭菌.冷却到60℃,再加100ml灭菌的170mmol/L KH2PO4/0。72 mol/L K2HPO4的溶液（2。31g的KH2PO4和12。54gK2HPO4溶在足量的水中,使终体积为100ml.高压灭菌或用0。22um的滤膜过滤除菌）.

2×YT培养基

将下列组分溶解在0.9L水中：

蛋白胨

酵母提取物

氯化钠

如果需要用1N NaOH（~1ml）调整pH至7。0,再补足水至1L。注：琼脂平板需添加琼脂粉12g/L，上层琼脂平板添加琼脂粉7g/L.

YPD培养基

将下列组分溶解在0.9L水中:

蛋白胨

酵母提取物

葡萄糖

用水补足体积为1L后，高压灭菌。建议在高压灭菌之前，对色氨酸营养缺陷型每升培养基添加1.6g色氨酸，因为YPD培养基是色氨酸限制型培养基。为了配制平板，需要在高压灭菌前加入20g琼脂粉。

四.常用抗生素

氨苄青霉素（ampicillin）（100mg/ml）

溶解1g氨苄青霉素钠盐于足量的水中,最后定容至10ml。分装成小份于-20℃贮存。常以25ug/ml～50ug/ml的终浓度添加于生长培养基。

羧苄青霉素（carbenicillin）（50mg/ml）

溶解0.5g羧苄青霉素二钠盐于足量的水中，最后定容至10ml。分装成小份于-20℃贮存.常以25ug/ml～50ug/ml的终浓度添加于生长培养基.

甲氧西林(methicillin）(100mg/ml）

溶解1g甲氧西林钠于足量的水中,最后定容至10ml。分装成小份于—20℃贮存.常以37.5ug/ml终浓度与100ug/ml氨苄青霉素一起添加于生长培养基。

卡那霉素（kanamycin）（10mg/ml）

溶解100mg卡那霉素于足量的水中，最后定容至10ml。分装成小份于-20℃贮存.常以10ug/ml～50ug/ml的终浓度添加于生长培养基。

氯霉素(chloramphenicol)（25mg/ml）

溶解250mg氯霉素足量的无水乙醇中，最后定容至10ml。分装成小份于-20℃贮存.常以12。5ug/ml～25ug/ml的终浓度添加于生长培养基.

链霉素（streptomycin）（50mg/ml）

溶解0.5g链霉素硫酸盐于足量的无水乙醇中,最后定容至10ml。分装成小份于-20℃贮存。常以10ug/ml～50ug/ml的终浓度添加于生长培养基。

20g

10g

20g

16g

10g

4ml

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreachDelegates, staff: Hello! in the run-up to the Spring Festival, we held one session of four staff representatives Conference 2013-workshop, full back in 2012, careful analysis of the current situation, discuss 2013 development plans. Here, on behalf of my company 2013 work reports to the General Assembly, for consideration. Pillar I, 2012 back in 2012, XX power companies adhere to the party\'s 17 great spirit for guidance, comprehensively implement the scientific concept of development, promoting cost-leadership strategy, standards, focus on implementation, lean management, continuously improve, smooth present safety situation of enterprise management, business management and control scientific and standardized, and the dedication of staff, manage a harmonious and democratic atmosphere of the good situation. Main indicators are as follows:-the battery indicator: power generation totaled 7.815 billion kWh, beyond the annual budget implementation capacity of 315

million kWh, an increase of 757 million kWh. Sales totaled 7.425 billion kWh, exceeding sales of 330 million kWh the annual Executive budget, an increase of 729 million kWh. --Security measures: unplanned outages 2.5 times. No personal injury accident occurred, no major accident and above, no major fire accidents without environmental pollution accidents, safety for three consecutive years to maintain stability to good posture. Business financial indicators: total profits of 255 million Yuan, beyond the annual budget of 207 million Yuan, beyond the Datang company index 41.89 million Yuan, an increase of 1.76 million Yuan, FCM assessment at grade four. --Energy: power supply standard coal completing 312.25 g/kWh, down 0.1 g/kWh; integrated auxiliary power consumption ratio in 5.12%, down 0.26%; pollutant emissions performance greatly reduced compared to last year, carbon 0.09 g/kWh, sulfur dioxide 0.104 g/kWh NOx 0.512 g/kWh; dust removaefficiency of more than 99.8%. --Reliability index: equivalent availability factor in 93.47%, increased 7.95% from a year earlier. Equivalent forced outage rate 0.08%, 0.16% reduction over the same period a year earlier. Major achievements: first, we should adhere to the two \"management system\" basis, strengthening technological research, strengthen hidden hazards control and intrinsic safety Enterprise construction took new steps. -The two \"management system\" for improvement. Focus on promoting the power of the company management system and the application and implementation of the safety loop five-star management system, improve the safety management system, realize the system of safety control. Further regulate security routines, safety supervision and management network role to play to achieve closed-loop. Strengthening the supervision and management of habitual violation of, strengthening the safety supervision of outsourcing contractors. Carried out in

spring and autumn of security inspections, flood control and inspection, safety production month, day supervision of production safety and the Olympic Games and other萘啶酮酸（nalidixic acid）（5mg/ml）

溶解50mg萘啶酮酸钠盐于足量的水中,最后定容至10ml。分装成小份于-20℃贮存。常以15ug/ml的终浓度添加于生长培养基。

四环素（tetracyyline）（10mg/ml）

溶解100mg四环素盐酸盐于足量的水中，或者将无碱的四环素溶于无水乙醇，定容至10ml。分装成小份用铝箔包裹装液管以免溶液见光,于-20℃贮存。常以10ug/ml～50ug/ml的终浓度添加于生长培养基。

activities, comprehensive and tamping Safety Foundation ... Troubleshooting, management mechanism, give full play to role of technical supervision and realization of troubleshooting, management, improved process management. This year completed the boiler lower header leakage, boiler pressure, a major risk management, completed 29 of great risks and 3 General problems of governance. Complete chemistry lab construction, thermal control, and complete the boiler scale integrated management, host shafting vibration of 10 scientific and technological projects, such as. Complete supercritical 630MW on-line simulation system development and application of circulating water MCC standby power transformation, the transformation of desulfurization waste water, the unit water supply system of comprehensive treatment and discharge valve modification of coal mill 5 key technological transformation projects, group health is improved. --Science and technology innovation is furtherincreased. Strengthen the characteristics of supercritical unit major issues, gradually clearing the particularity of supercritical unit and regularity. Developed motor oil time management, switch action times, statistics, coal-aided measurement software, improves the production level of lean management. Increased investment in science and technology, reporting science and technology projects and 14 technical project total cost percentage of the total annual production output of 0.25%. \"Large-scale coal-fired power plant flue gas desulfurization, denitrification complete development and application of key technologies\" project, won the national science and technology progress second prize. 630MW supercritical units optimized control strategies and the 630MW development and application of on-line simulation system for supercritical units, supercritical 600MW units of turbine driven boiler feed pump set of comprehensive treatment of defects Datang technology respectively one or two and

third. Meanwhile, information technology achievements, the company was named \"China power information technology benchmarking enterprises.\" --Repair and maintenance has improved further. Modify the inspection standards and standards on a regular basis, standardizing work procedures, checking and inspection project. Deepening the BFS++ system, and implements maintenance information shared. Reorganizing RB logic again, and ensure the success of the RB. Innovating the mechanism of maintenance management, implemented a project manager system. Successful completion of two autonomous maintenance, reliability improved steadily. Implementing two c-level maintenance, project themselves 48.7% and 42.3%, respectively. Accomplish two circulating pumps repair and overhaul of four Mills, maintenance teams to get exercise. Promote the work of energy saving and consumption reducing, complete the unit energy consumption diagnosis, plant water balancetest, 10 energy-saving projects. Second, we should adhere to \"three\" on the economic benefits of improving, outreach